抗生素
细菌外膜
流出
微生物学
化学
细菌
抗药性
抗菌剂
药理学
药品
生物化学
生物
大肠杆菌
遗传学
基因
作者
Weiwei Huang,Qishu Zhang,Weiran Li,Mingcui Yuan,Jingxian Zhou,Liangqun Hua,Yongjun Chen,Chao Ye,Yanbing Ma
标识
DOI:10.1016/j.jconrel.2019.11.017
摘要
Conventionally used antibiotics are present in low concentrations at the infection site and require multiple administrations to sustain a continuous bactericidal effect, which not only increases their systemic toxicity but also results in bacterial drug resistance. In this study, we first identified an interesting drug resistance mechanism mediated by bacterial outer membrane vesicles (OMVs) and then designed novel antibiotic-loaded OMVs using this mechanism. We show that these antibiotic-loaded OMVs can effectively enter and kill pathogenic bacteria in vitro. In a mouse model of intestinal bacterial infection, one low-dose oral administration of antibiotic-loaded OMVs showed that the drug was retained in the intestine for 36 h, and no systemic spread was detected 12 h after drug administration. The antibiotic-loaded OMVs significantly reduced the bacterial load in the small intestine and feces of infected mice. Safety experiments confirmed that the antibiotic-loaded OMVs had excellent biocompatibility. This study extends the application range of OMVs and provides new ideas for the development of antibacterial drugs.
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