着丝粒
顺序装配
基因组
染色质免疫沉淀
生物
计算生物学
DNA测序
遗传学
DNA
基因
染色体
发起人
基因表达
转录组
作者
Miaomiao Han,Yae Yang,Muqing Zhang,Kai Wang
出处
期刊:Methods
[Elsevier BV]
日期:2021-03-01
卷期号:187: 54-56
被引量:3
标识
DOI:10.1016/j.ymeth.2020.09.006
摘要
The assembly of centromeric regions has become one of the most intractable tasks in whole-genome sequencing due to the enrichment of highly repetitive DNA sequences in most eukaryotic centromeres. Here, we describe a method used to identify centromeric DNAs through chromatin immunoprecipitation and sequencing (ChIP-seq). By mapping ChIP-seq reads, centromeric regions can be indicated in genome assemblies. We demonstrated that the assembly quality of centromeres obtained using ChIP-seq mapping can reflect and indicate the quality of a whole-genome assembly. We discuss an expected 'high-quality' centromere assembly obtained via centromere ChIP-seq mapping.
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