粒体自噬
ATG8型
线粒体
细胞生物学
线粒体分裂
波姆裂殖酵母
生物
自噬
模式生物
酵母
酿酒酵母
遗传学
基因
细胞凋亡
作者
Tomoyuki Fukuda,Yuki Ebi,Toyohei Saigusa,Kentaro Furukawa,Shunichi Yamashita,Keiichi Inoue,Daiki Kobayashi,Yutaka Yoshida,Tomotake Kanki
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2020-11-03
卷期号:9
被引量:30
摘要
Degradation of mitochondria through mitophagy contributes to the maintenance of mitochondrial function. In this study, we identified that Atg43, a mitochondrial outer membrane protein, serves as a mitophagy receptor in the model organism Schizosaccharomyces pombe to promote the selective degradation of mitochondria. Atg43 contains an Atg8-family-interacting motif essential for mitophagy. Forced recruitment of Atg8 to mitochondria restores mitophagy in Atg43-deficient cells, suggesting that Atg43 tethers expanding isolation membranes to mitochondria. We found that the mitochondrial import factors, including the Mim1–Mim2 complex and Tom70, are crucial for mitophagy. Artificial mitochondrial loading of Atg43 bypasses the requirement of the import factors, suggesting that they contribute to mitophagy through Atg43. Atg43 not only maintains growth ability during starvation but also facilitates vegetative growth through its mitophagy-independent function. Thus, Atg43 is a useful model to study the mechanism and physiological roles, as well as the origin and evolution, of mitophagy in eukaryotes.
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