生物
16S核糖体RNA
放线菌
二氨基酸
诺卡氏菌科
菌丝体
放线菌门
微生物学
化学分类学
二氨基二甲酸
拉伤
细菌
基因
生物化学
植物
链霉菌
分类学(生物学)
基因序列
遗传学
解剖
作者
Claudia Dalmastri,Luciano Gastaldo,Francesca Berini,Flavia Marinelli,Giorgia Letizia Marcone
标识
DOI:10.1099/ijsem.0.004348
摘要
The filamentous actinomycete that produces the antibiotic GE23077 was isolated by the Lepetit Research Group from a soil sample collected in Thailand, and it was classified as a member of the genus Actinomadura on the basis of its morphology and cell-wall composition. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain formed a distinct monophyletic line within the genus Actinomadura, and it was most closely related to Actinomadura bangladeshensis DSM 45347 T (99.31 % similarity) and Actinomadura mexicana DSM 44485 T (98.94 %). The GE23077-producing strain formed an extensively branched, non-fragmented vegetative mycelium; no pseudosporangia were formed and the arthrospores were organized in slightly twisted chains. The cell wall contained meso -2,6-diaminopimelic acid and the diagnostic sugar was madurose. The predominant menaquinone was MK-9(H 6 ), with minor amounts of MK-9(H 8 ) and MK-9(H 4 ). The diagnostic phospholipids were phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were C 16 : 0 and tuberculostearic acid (10-methyloctadecanoic acid), followed by minor amounts of C 18:1 ω9 c , C 16:1 ω7 c and 10-methylheptadecanoic acid. The genomic DNA G+C content was 71.77 mol%. Significant differences in the morphological, chemotaxonomic and biochemical data, and the low DNA–DNA relatedness between the GE23077-producing strain and closely related type strains clearly demonstrate that it represents a novel species of the genus Actinomadura , for which the name Actinomadura lepetitiana sp. nov. is proposed. The type strain is NRRL B-65521 T (=LMG 31258 T =DSM 109019 T ).
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