降钙素原
化学
检出限
色谱法
辣根过氧化物酶
生物标志物
免疫磁选
酶
生物化学
败血症
免疫学
生物
作者
Yun Zhang,Xiaohui Si,Miaomiao Zhang,Xue Yang,Huan Yuan,Xiangpeng Wang,Yang Zhang,Hui Wang
标识
DOI:10.1080/00032719.2018.1482312
摘要
Human procalcitonin is an early diagnostic biomarker for sepsis and bacterial infections and can be used in distinguishing bacterial infections from viral infections. In this study, a colorimetric sensing platform for the rapid determination of procalcitonin was developed. The approach involves the capture of procalcitonin by immunomagnetic beads, and a detection antibody labeled with horseradish peroxidase to perform sandwich format, where it catalyzes the oxidation of 3,3′,5,5′–tetramethylbenzidine to produce the colorimetric signal. Under the optimal conditions, a detection limit of 0.04 ng/mL (3σ) was obtained within the calibration range 0.1–10 ng/mL. The proposed method was performed in less than 90 min and exhibited good specificity without interferences from other biomarkers including C-reactive protein and human serum albumin. Overall, the proposed method provided a new alternative strategy for procalcitonin detection due to its sensitive, rapid, specific, and simple characteristics. This method is suitable for rapid screening of various biomedical targets.
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