化学
过氧化氢酶
膜
膜流动性
过氧化物酶
生物化学
愈创木酚
脂质过氧化
谷胱甘肽还原酶
抗氧化剂
酶
膜脂
电子顺磁共振
小粒咖啡
酶分析
生物物理学
谷胱甘肽过氧化物酶
植物
生物
核磁共振
物理
作者
Cristina Generosa de Senna Queiroz,Antonio Alonso,Marcos Mares-Guia,Arinos Magalhães
出处
期刊:Biologia Plantarum
[Institute of Experimental Botany]
日期:1998-04-01
卷期号:41 (3): 403-413
被引量:73
标识
DOI:10.1023/a:1001802528068
摘要
Exposure of coffee to low temperatures caused growth inhibition, changes in metabolic rates, and membrane alterations. Root tissue exposed to 10 °C evolved significantly lower rates of metabolic heat compared with controls grown at 25 °C, and the values were closely associated with the observed root growth inhibition. Electron paramagnetic resonance spectra of intact tissues showed that the spin probe 5-doxylstearic acid was capable to intercalate within the cellular membrane lipids. Indeed, at the depth of the 5th carbon atoms of the alkyl chains, the nitroxide radical detected more rigid membranes in seedlings exposed to 10 °C compared with 25 °C treated samples. Ascorbate peroxidase and catalase activities did not show appreciable changes under chilling conditions, while guaiacol peroxidase activity increased 55 % compared to the control. On the other hand, glutathione reductase activity decreased, in parallel to a significant decline in the capacity to reduce triphenyl-tetrazolium. Our results showed a marked correlation between lipid peroxidation and root tissue damage, which seemed to be associated with increased membrane rigidity.
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