A large, switchable optical clearing skull window for cerebrovascular imaging

颅骨 窗口(计算) 清理 生物医学工程 材料科学 医学 计算机科学 外科 财务 操作系统 经济
作者
Chao Zhang,Wei Feng,Yanjie Zhao,Tingting Yu,Pengcheng Li,Tonghui Xu,Qingming Luo,Dan Zhu
出处
期刊:Theranostics [Ivyspring International Publisher]
卷期号:8 (10): 2696-2708 被引量:71
标识
DOI:10.7150/thno.23686
摘要

Rationale: Intravital optical imaging is a significant method for investigating cerebrovascular structure and function. However, its imaging contrast and depth are limited by the turbid skull. Tissue optical clearing has a great potential for solving this problem. Our goal was to develop a transparent skull window, without performing a craniotomy, for use in assessing cerebrovascular structure and function. Methods: Skull optical clearing agents were topically applied to the skulls of mice to create a transparent window within 15 min. The clearing efficacy, repeatability, and safety of the skull window were then investigated. Results: Imaging through the optical clearing skull window enhanced both the contrast and the depth of intravital imaging. The skull window could be used on 2-8-month-old mice and could be expanded from regional to bi-hemispheric. In addition, the window could be repeatedly established without inducing observable inflammation and metabolic toxicity. Conclusion: We successfully developed an easy-to-handle, large, switchable, and safe optical clearing skull window. Combined with various optical imaging techniques, cerebrovascular structure and function can be observed through this optical clearing skull window. Thus, it has the potential for use in basic research on the physiopathologic processes of cortical vessels.
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