Frequency of Viable Mutants in M2 and M3 Generation of Black Gram [Vigna mungo (L.) Hepper] Through Induced Mutation

The present investigation was conducted during the Kharif season of 2013, seeds of black gram [Vingo mungo (L.) Hepper] variety VBN 4 and ACM 07002 were exposed to gamma rays doses at 200, 250, 300 and 350 Gy and EMS (Ethyl Methane Sulphonate) doses at 20, 30 and 40 mM. Wide ranges of viable mutants were isolated in M2 and M3 generation. The mutational change in each quantitative trait can be large or small. Such changes in both macro and micro mutation are of significance in breeding depending on the characters involved. Among the seven doses of treatment in gamma rays and EMS, the dose 350 Gy and 40 mM in VBN 4 and 300 Gy and 40 mM in ACM 07002 registered highest frequency of viable mutants in M2 generation and similarly in M3 generation highest viable mutants were recorded in doses of 350Gy and 30 mM in VBN 4 and 350 Gy and 20 mM in ACM 07002