光遗传学
光纤
光学
纤维
材料科学
数值孔径
单模光纤
神经科学
波长
计算机科学
生物医学工程
物理
生物
医学
复合材料
作者
Marco Pisanello,Gil Mandelbaum,Ian Antón Oldenburg,Leonardo Sileo,Jeffrey E. Markowitz,Ralph E. Peterson,Andrea Della Patria,Trevor M. Haynes,Mohamed S. Emara,Barbara Spagnolo,Sandeep Robert Datta,Massimo De Vittorio,Bernardo L. Sabatini
摘要
The authors demonstrate that optical fibers with tapered tips can homogenously illuminate either elongated brain structures or dynamically selected subregions. Tapered fibers achieve efficient optogenetic stimulation in vivo with minimal tissue damage. In addition, a single fiber can deliver light of multiple wavelengths to independently controlled regions. Optogenetics promises precise spatiotemporal control of neural processes using light. However, the spatial extent of illumination within the brain is difficult to control and cannot be adjusted using standard fiber optics. We demonstrate that optical fibers with tapered tips can be used to illuminate either spatially restricted or large brain volumes. Remotely adjusting the light input angle to the fiber varies the light-emitting portion of the taper over several millimeters without movement of the implant. We use this mode to activate dorsal versus ventral striatum of individual mice and reveal different effects of each manipulation on motor behavior. Conversely, injecting light over the full numerical aperture of the fiber results in light emission from the entire taper surface, achieving broader and more efficient optogenetic activation of neurons, compared to standard flat-faced fiber stimulation. Thus, tapered fibers permit focal or broad illumination that can be precisely and dynamically matched to experimental needs.
科研通智能强力驱动
Strongly Powered by AbleSci AI