生物
基因
转化(遗传学)
转基因
耐旱性
转基因作物
胡杨
农杆菌
cDNA末端的快速扩增
互补DNA
亚科
棉属
植物
分子生物学
遗传学
分子克隆
作者
Jin Bo Li,Xue Dong,Zhi Liu,Yong Liang Li,Pei Yang Yang,Fei Tao,Liang Zhao,Shi Gang Li,Lin Du,Ji Shao,Yunpeng Wu
出处
期刊:Protein and Peptide Letters
[Bentham Science]
日期:2016-04-11
卷期号:23 (5): 450-458
被引量:4
标识
DOI:10.2174/0929866523666160314153212
摘要
A desert-grown Halimodendron halodendron ethylene-responsive element binding factor gene (HhERF2), which encodes a 245 amino acids protein containing a conserved AP2/EREBP domain, was isolated through the rapid amplification cDNA end (RACE) method. Sequence and phylogenetic analysis indicated that HhERF2 was classified into the B-2 group of ERF subfamily. Semiquantitative RT-PCR showed that HhERF2 was greatly induced by treatments with high-salt, drought and low temperature in H. halodendron seedlings. The expression vector containing HhERF2 and Populus euphratica dehydration- responsive element binding protein (PeDREB2a) genes driven by rd29A promoter was constructed and transferred into cotton (Gossypium hirsutum L.) by non-tissue culture Agrobacterium-mediated genetic transformation system. The transformation and expression of HhERF2 and PeDREB2a were identified by PCR and RT-PCR. Analyses of physiological function indicated that transgenic cottons had improved seeds germination, tolerance to drought and highsalt stresses. Agronomic evaluation in the field exhibited that transgenic lines presented a dwarf phenotype and improved further in the yield and characters. These results demonstrated that overexpressed both HhERF2 and PeDREB2a transcription factor genes in cotton induced elevated tolerance to drought and high-salt stresses.
科研通智能强力驱动
Strongly Powered by AbleSci AI