抗原呈递
细胞生物学
巨噬细胞
U937电池
免疫系统
树突状细胞
抗原提呈细胞
生物
滤泡树突状细胞
免疫学
细胞培养
THP1细胞系
吞噬作用
电池类型
单核细胞
化学
体外
细胞
T细胞
遗传学
作者
Wasaporn Chanput,Vera Maria Peters,Harry J. Wichers
出处
期刊:Springer eBooks
[Springer Nature]
日期:2015-01-01
卷期号:: 147-159
被引量:32
标识
DOI:10.1007/978-3-319-16104-4_14
摘要
Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes and their macrophage and dendritic-cell progeny serve three main functions in the immune system. These are phagocytosis, antigen presentation, and cytokine production. THP-1 and U937 are (pro-) monocytic cell lines that can, also in vitro, be differentiated into either various types of macrophages or into dendritic cells. This chapter describes how to grow THP-1, resp. U937 cells, how to differentiate these into more specialised phenotypes such as various macrophage types or dendritic cells, how to read-out their responses to stimuli and it gives examples of how such cell lines have been used into studying the effects of food compounds.
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