双功能
试剂
分子内力
戊二醛
化学
酶
组合化学
磷酸果糖激酶2
固定化酶
马来酰亚胺
有机化学
催化作用
作者
Roberto Fernández‐Lafuente,Cristina M. Rosell,Verónica Rodrı́guez,José M. Guisán
标识
DOI:10.1016/0141-0229(94)00090-e
摘要
A original strategy to obtain intramolecular crosslinking is discussed. This strategy consisted of three consecutive steps to direct the reaction to the formation of intramolecular crosslinks: (a) enzyme are partially modified with the bifunctional reagent in a very controlled fashion; (b) the excess of reagent is removed; and (c) the modified enzyme is incubated long-term to allow a crosslinking reaction without the competition of additional single-point modifications. In this way, we were able to obtain interesting stabilizations of immobilized derivatives of penicillin G acylase using glutaraldehyde as a crosslinking reagent under very controlled conditions.
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