谷胱甘肽
化学
吸光度
组合化学
谷胱甘肽S-转移酶
纳米技术
酶
色谱法
生物化学
材料科学
作者
Ying Wang,Jie Sun,Qingran Yang,Wenbo Lu,Yan Li,Jian Dong,Weiping Qian
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2015-01-01
卷期号:140 (22): 7578-7585
被引量:8
摘要
The developed method for monitoring GST, an important drug metabolic enzyme, could greatly facilitate researches on relative biological fields. In this work, we have developed a SERS technique to monitor the absorbance behaviour of 6-mercaptopurine (6-MP) and its glutathione-S-transferase (GST)-accelerated glutathione (GSH)-triggered release behaviour on the surface of gold nanoflowers (GNFs), using the GNFs as excellent SERS substrates. The SERS signal was used as an indicator of absorbance or release of 6-MP on the gold surface. We found that GST can accelerate GSH-triggered release behaviour of 6-MP from the gold surface. We speculated that GST catalyzes nucleophilic GSH to competitively bind with the electrophilic substance 6-MP. Experimental results have proved that the presented SERS protocol can be utilized as an effective tool for accessing the release of anticancer drugs.
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