Cloning and sequence analysis of Actin gene fragment from Cajanus cajan.

A pair of primers,Primer 1 and Primer 2,was designed according to the conserved sequences of the Actin gene from Glycine max.The Actin gene fragment was amplified by RT-PCR based on total RNA of Cajanus cajan leaves and then cloned into PMD-18T vector.The positive clone was identified via PCR and then sequenced.The sequence analysis showed that the Actin gene fragment from Cajanus cajan contains 302 bp and 100 amino acids.The homology of nucleotide sequence and amino acid sequence of the obtained sequence is above 89% and 90% compared with the nucleotide sequence and amino acid sequence of Actin gene of other plants registered in GenBank.The nucleotide sequence homology of the obtained sequence is up to 94% compared with the nucleotide sequence of Actin gene of Glycine max.