微流控
炸薯条
吞吐量
循环肿瘤细胞
体积流量
样品(材料)
频道(广播)
分离(统计)
分类
微流控芯片
材料科学
计算机科学
色谱法
纳米技术
化学
癌症
机械
电信
物理
内科学
机器学习
医学
程序设计语言
无线
转移
作者
Xiaohan Xu,Xiwei Huang,Jingjing Sun,J. Chen,Guangjun Wu,Yuan Yao,Nan Zhou,S. Wang,Lingling Sun
标识
DOI:10.34133/2022/9829287
摘要
Whether for cancer diagnosis or single-cell analysis, it remains a major challenge to isolate the target sample cells from a large background cell for high-efficiency downstream detection and analysis in an integrated chip. Therefore, in this paper, we propose a 3D-stacked multistage inertial microfluidic sorting chip for high-throughput enrichment of circulating tumor cells (CTCs) and convenient downstream analysis. In this chip, the first stage is a spiral channel with a trapezoidal cross-section, which has better separation performance than a spiral channel with a rectangular cross-section. The second and third stages adopt symmetrical square serpentine channels with different rectangular cross-section widths for further separation and enrichment of sample cells reducing the outlet flow rate for easier downstream detection and analysis. The multistage channel can separate 5 μ m and 15 μ m particles with a separation efficiency of 92.37% and purity of 98.10% at a high inlet flow rate of 1.3 mL/min. Meanwhile, it can separate tumor cells (SW480, A549, and Caki-1) from massive red blood cells (RBCs) with a separation efficiency of >80%, separation purity of >90%, and a concentration fold of ~20. The proposed work is aimed at providing a high-throughput sample processing system that can be easily integrated with flowing sample detection methods for rapid CTC analysis.
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