葡萄籽提取物(GSE)有效成分的分析

As the polyphenols of the Grape Seeds Extracts (GSE) contains proanthocyanidins and mononeric phenols, the difference between total amount of polyphenols (TAPP) and monomeric phenols (TAMP) could represent the content of proanthocyanidins (CP) in GSE. In this manuscript, TAPP of GSE was determined by the Folin-Ciocalteumethod, expressed as Gallic Acid Equivalents, and TAMP determined by HPLC. The concentration of TAPP in GSE sample which made by this study was 90.5%, was higher 2.4% than that of the control (88.3%, Jianfeng GSE, Tianjin). For the TAMP case, the content of gallic acid and catechin were higher, but the content of epicatechin and epicatechin gallate were lower than that of the control. For CP, no significant difference between the two GSEs sample was detected, was 74.6% and 75.8%, respectively.