作者
Mingzhou Li,Lei Chen,Shilin Tian,Yu Lin,Qianzi Tang,Xuming Zhou,Diyan Li,Carol K.L. Yeung,Tiandong Che,Long Jin,Yuhua Fu,Jideng Ma,Xun Wang,Anan Jiang,Jing Lan,Qi Pan,Yingkai Liu,Zonggang Luo,Zhanbao Guo,Haifeng Liu,Li Zhu,Surong Shuai,Guoqing Tang,Jianmin Zhao,Yanzhi Jiang,Lin Bai,Shunhua Zhang,Miaomiao Mai,Changchun Li,Dawei Wang,Yiren Gu,Guosong Wang,Hongfeng Lu,李岩,Haihao Zhu,Zongwen Li,Ming Li,Vadim N. Gladyshev,Zhi Jiang,Shuhong Zhao,Jinyong Wang,Ruiqiang Li,Xuewei Li
摘要
Uncovering genetic variation through resequencing is limited by the fact that only sequences with similarity to the reference genome are examined. Reference genomes are often incomplete and cannot represent the full range of genetic diversity as a result of geographical divergence and independent demographic events. To more comprehensively characterize genetic variation of pigs ( Sus scrofa ), we generated de novo assemblies of nine geographically and phenotypically representative pigs from Eurasia. By comparing them to the reference pig assembly, we uncovered a substantial number of novel SNPs and structural variants, as well as 137.02-Mb sequences harboring 1737 protein-coding genes that were absent in the reference assembly, revealing variants left by selection. Our results illustrate the power of whole-genome de novo sequencing relative to resequencing and provide valuable genetic resources that enable effective use of pigs in both agricultural production and biomedical research.