中国仓鼠卵巢细胞
生物
细胞生物学
细胞生长
异源的
细胞
糖基化
蛋白质生物合成
翻译(生物学)
生物制药
生物化学
二氢叶酸还原酶
细胞周期
细胞培养
分子生物学
信使核糖核酸
基因
遗传学
作者
Ningning Xu,Chao Ma,Jianfa Ou,Wanqi Sun,Lufang Zhou,Hui Hu,Margaret Liu
标识
DOI:10.1016/j.bej.2017.05.007
摘要
Chinese hamster ovary (CHO)1 cells have been widely used to express heterologous genes and produce therapeutic proteins in biopharmaceutical industry. Different CHO host cells have distinct cell growth rates and protein expression characteristics. In this study, the expression of about 1307 host proteins in three sublines, i.e. CHO K1, CHO S and CHO/dihydrofolate reductase (dhfr)−, were investigated and compared using proteomic analysis. The proteins involved in cell growth, glycolysis, tricarboxylic acid cycle, transcription, translation and glycosylation were quantitated using Liquid chromatography tandem-mass spectrometry (LC–MS/MS). The key host cell proteins that regulate the kinetics of cell growth and the magnitude of protein expression levels were identified. Furthermore, several rational cell engineering strategies on how to combine the desired features of fast cell growth and efficient production of therapeutic proteins into one new super CHO host cell have been proposed.
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