Dendrobium huoshanense is a precious medicinal plant belonging to Dendrobium of Orchidaceae. It is a special medicinal material and extremely scarce in Huoshan county, Anhui province. At present, D. huoshanense has been greatly protected, which also makes it possible to industrialize relying on tissue culture and artificial cultivation technology. Three main planting methods were utilized for cultivating D. huoshanense including facility cultivation, under forest cultivation and simulative habitat cultivation. Firstly, the three cultivation modes and technical characteristics of D. huoshanense were compared and analyzed, and it was found that the ecological of D. huoshanense cultivated in the simulated was closer to that of wild D. huoshanense. Secondly, based on comparing the characters and of three cultivation modes, the results showed that the of D. huoshanense cultivated in simulated was more similar to that of grasshopper thigh recorded in Bencao Jing Jizhu, and its was better than that of facilities and under forest cultivation. The comprehensive benefit comparison of three modes showed that the simulated cultivation had income, the lowest input-output ratio and significant economic benefit. The of cultivated D. huoshanense was further evaluated from four aspects of excellent environment excellent shape high quality excellent effect, which summarized the comprehensive advantages of simulative habitat cultivation of D. huoshanense as follows: the original habitat and site of simulated wild D. huoshanense, the closer to the wild, the more content of main medicinal components, and higher economic benefit and better efficacy. The of D. huoshanense was improved by the use of simulative habitat cultivation, which has practical significance to guide its large-scale cultivation.