生物相容性
聚己内酯
生物活性玻璃
组织工程
生物医学工程
材料科学
脚手架
复合数
粘附
骨组织
扫描电子显微镜
复合材料
模拟体液
聚合物
医学
冶金
作者
Mauro Petretta,Alessandro Gambardella,Marco Boi,Matteo Berni,Carola Cavallo,Gregorio Marchiori,Maria Cristina Maltarello,Devis Bellucci,Milena Fini,Nicola Baldini,Brunella Grigolo,Valeria Cannillo
出处
期刊:Biology
[MDPI AG]
日期:2021-05-04
卷期号:10 (5): 398-398
被引量:59
标识
DOI:10.3390/biology10050398
摘要
Polycaprolactone (PCL) is widely used in additive manufacturing for the construction of scaffolds for tissue engineering because of its good bioresorbability, biocompatibility, and processability. Nevertheless, its use is limited by its inadequate mechanical support, slow degradation rate and the lack of bioactivity and ability to induce cell adhesion and, thus, bone tissue regeneration. In this study, we fabricated 3D PCL scaffolds reinforced with a novel Mg-doped bioactive glass (Mg-BG) characterized by good mechanical properties and biological reactivity. An optimization of the printing parameters and scaffold fabrication was performed; furthermore, an extensive microtopography characterization by scanning electron microscopy and atomic force microscopy was carried out. Nano-indentation tests accounted for the mechanical properties of the scaffolds, whereas SBF tests and cytotoxicity tests using human bone-marrow-derived mesenchymal stem cells (BM-MSCs) were performed to evaluate the bioactivity and in vitro viability. Our results showed that a 50/50 wt% of the polymer-to-glass ratio provides scaffolds with a dense and homogeneous distribution of Mg-BG particles at the surface and roughness twice that of pure PCL scaffolds. Compared to pure PCL (hardness H = 35 ± 2 MPa and Young’s elastic modulus E = 0.80 ± 0.05 GPa), the 50/50 wt% formulation showed H = 52 ± 11 MPa and E = 2.0 ± 0.2 GPa, hence, it was close to those of trabecular bone. The high level of biocompatibility, bioactivity, and cell adhesion encourages the use of the composite PCL/Mg-BG scaffolds in promoting cell viability and supporting mechanical loading in the host trabecular bone.
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