Abstract A system was designed to imitate on a small scale the separation process of wheat into starch and gluten. The system provides information on gluten coagulation, on yields, purity and recovery of gluten and starch, and on losses of soluble protein and other soluble matter in waste water. The performance of the system is demonstrated with the results of the separation of four wheat varieties of different baking quality. Using these results the accuracy, reproducibility and resolution of the system are discussed.