微小隐孢子虫
体外
隐孢子虫
药品
高通量筛选
生物
微生物学
药理学
生物信息学
生物化学
粪便
作者
Haili Zhang,Guangxi Zhu
出处
期刊:Methods in molecular biology
日期:2019-08-27
卷期号:: 319-334
被引量:17
标识
DOI:10.1007/978-1-4939-9748-0_18
摘要
An effective method to quantify the parasite loads is the key to the evaluation of anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening (HTS) of drugs against Cryptosporidium parvum in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of C. parvum in vitro.
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