癌症研究
细胞周期蛋白D1
细胞生长
基因沉默
细胞周期
流式细胞术
生物
免疫组织化学
宫颈上皮内瘤变
癌症
宫颈癌
分子生物学
医学
内科学
免疫学
基因
生物化学
遗传学
作者
Ying Wang,Sai Han,Xuewu You,Xuejiao Shi,Ling Lü,Yu Sun,Yana Ma,Qiuhong Qian,Hongli Liu,Baoxia Cui,Youzhong Zhang
摘要
It is unclear whether low density lipoprotein receptor-related protein 11 (LRP11), a newly found lipoprotein receptor regulatory protein, has the carcinogenic effects in cervical cancer.Bioinformatics analysis, immunohistochemical (IHC) staining and evaluation, cell proliferation assay, flow cytometry, transwell migration and invasion assays, Western blotting, growth of LRP11-silenced cells in athymic nude mice were performed in this research.We found that LRP11 expression was higher in high-grade squamous intraepithelial lesions (HSIL) and cervical cancer tissue than in normal cervix, and high expression of LRP11 was associated with differentiation degree (P=0.0266), indicating poor prognosis (P=0.0210). The silencing of LRP11 in SiHa and CaSki cell lines inhibited cell proliferation, reduced migration and invasion and suppressed cell growth in nude mice, which possibly related to cell cycle protein regulation of CDK 2/4, cyclin D1/E1, MMP-2/9, and VEGF. Furthermore, LRP11 showed substantial positive correlation with P16 in vivo and in vitro.LRP11 plays important roles in proliferation, migration and invasion, with the potential to be a useful prognostic marker and therapeutic target for patients with HSIL and cervical cancer.
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