化学
空白
生物标志物
代谢组学
色谱法
鉴定(生物学)
生物化学
机械工程
植物
工程类
生物
作者
Yuyao Yuan,Huajie Song,Juntuo Zhou,Yuxin Yin
标识
DOI:10.1021/acs.analchem.3c01864
摘要
As the first step of metabolomic analysis in biomarker identification studies, various types of blood collection tubes are used in clinical practice. However, little attention is paid to potential contamination caused by the blank tube itself. Here, we evaluated small molecules in blank EDTA plasma tubes through LC-MS-based untargeted metabolomic analysis and identified small molecules with markedly varied levels among different production batches or specifications. Our data demonstrate possible contamination and data interference caused by blank EDTA plasma tubes when employing large clinical cohorts for biomarker identification. Therefore, we propose a workflow of filtering metabolites in blank tubes prior to statistical analysis to improve the fidelity of biomarker identification.
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