Role of lignin in synergistic digestibility improvement of wheat straw by novel alkaline deep eutectic solvent and tetrahydrofuran pretreatment

A novel efficient pretreatment system containing alkaline deep eutectic solvent (DES) and tetrahydrofuran (THF) was developed in the present study. Under pretreatment conditions of 160 ℃ and 1 h, DES-THF pretreatment was more efficient (81.61%) in cellulose digestibility improvement than DES (choline chloride/monoethanolamine, 67.54%). To further explore lignin structural transformation and lignin-cellulase interaction after pretreatment, milled wood lignin (MWL) was extracted and characterized. Compared with DES-MWL, DES-THF-MWL showed an increased carboxyl group content (24.0%) and decreased condensed phenolic hydroxyl content (9.1%). In DES-MWL, β-O-4 content was 21.79%, while in DES-THF-MWL, β-O-4 accounted for 45.45%, indicating that the addition of THF alleviated cleavage of β-O-4 alkyl ether bonds. Fluorescence emission spectroscopy results showed that quenching mechanism of DES-THF-MWL and cellulase was dynamic, which was different from other lignin. Compared with DES-MWL, decreased K