聚二甲基硅氧烷
气泡
帕利烯
炸薯条
蒸汽压
液体气泡
材料科学
复合材料
化学
工程类
聚合物
机械
电气工程
物理
有机化学
作者
Shiyuan Gao,Tingting Xu,Lei Wu,Xiaoyue Zhu,Xuefeng Wang,Ying Chen,Gang Li,Xinxin Li
出处
期刊:Biosensors
[MDPI AG]
日期:2024-02-21
卷期号:14 (3): 114-114
摘要
In a chamber-based digital PCR (dPCR) chip fabricated with polydimethylsiloxane (PDMS), bubble generation in the chambers at high temperatures is a critical issue. Here, we found that the main reason for bubble formation in PDMS chips is the too-high saturated vapor pressure of water at an elevated temperature. The bubbles should be completely prevented by reducing the initial pressure of the system to under 13.6 kPa to eliminate the effects of increased-pressure water vapor. Then, a cavity was designed and fabricated above the PCR reaction layer, and Parylene C was used as a shell covering the chip. The cavity was used for the negative generator in sample loading, PDMS degassing, PCR solution degassing in the digitization process and water storage in the thermal reaction process. The analysis was confirmed and finally achieved a desirable bubble-free, fast-digitization, valve-free and no-tubing connection dPCR.
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