Portable smartphone-assisted ratiometric fluorescence sensor for visual detection of glucose

Fluorescence-based visual assays have sparked tremendous attention in on-site detection due to their obvious color gradient changes and high sensitivity. In this study, a novel emission wavelength shift-based visual sensing platform is constructed to detect glucose based on the oxidation of Rhodamine B (RhB). MnO2 nanosheets (MnO2 NS) with strong oxidizing properties were introduced to oxidize RhB, which resulted in a blue shift in the emission wavelength, and a visual color changed of the fluorescence from orange-red to green. The oxidation reaction could be inhibited via reducing and destroying MnO2 NS by H2O2, which was produced by the oxidizing procedure of glucose in the presence of glucose oxidase (GOx). A series of wavelength shifts and fluorescence color variations appeared with the addition of various amounts of glucose. A ratiometric fluorescence glucose sensor with a lowest recorded concentration of 0.25 μM was developed. Meanwhile, test paper-based assays integrated with the smartphone platform were established for the sensing of glucose by means of the significant fluorescence color changes, offering a reliable, sensitive, and portable on-site assay of glucose.