Regulation of baking quality and starch digestibility in whole wheat bread based on β-glucans and protein addition strategy: Significance of protein-starch-water interaction in dough

Whole wheat bread has high nutritional value but is characterized by inferior quality and a high glycemic index. Studies have shown that adding β-glucans and protein can improve bread quality. This study investigated the effects of added oat β-glucan, barley β-glucan, or yeast β-glucan on protein synergy and whole wheat dough and bread quality. The mixing properties, rheological properties, and scanning electron microscopy observations showed that the addition of β-glucan promoted the formation of gluten networks, while the synergy between the wheat proteins and β-glucan resulted in a more robust and stable gluten network and a stronger physical starch envelope. Rapid visco-analysis and thermal property evaluations showed that β-glucan addition inhibited the thermal degradation, gelatinization, and retrogradation of starch. Based on the bread quality results, it was found the β-glucan could cause some damage to the bread baking quality. For example, the hardness of samples with oats, barley, and yeast increased to 881.69 g, 952.97 g, and 631.75 g, respectively, compared to samples without β-glucan (317.49 g), whereas the inclusion of yeast β-glucan proved to be less detrimental. Protein and β-glucan both reduced starch digestion to some degree, and showed better synergistic effects, with the lowest estimated glycemic index of 70.08 observed in bread containing added yeast β-glucan and protein. Therefore, yeast β-glucan and protein mixtures could be selected as viable formulations for enhancing the quality of whole wheat bread.