适体
室内生物气溶胶
化学
检出限
纳米传感器
纳米技术
生物气溶胶
环境化学
分子生物学
色谱法
材料科学
生物
有机化学
气溶胶
作者
Rui Zhang,Pu Wang,Yangyang Chang,Meng Liu
标识
DOI:10.1021/acs.analchem.2c03460
摘要
Bioaerosols could carry and spread harmful microorganisms, thus posing a continuous threat to human beings and livestock health. Early warning and management are crucial for controlling the spread of bioaerosols. Herein, we developed a split aptamer (SA)-based electrochemical nanosensor chip (denoted SAE-nChip) for rapid and sensitive detection of adenosine triphosphate (ATP) in bioaerosols. The platform features two components: split DNA aptamers for their ability to bind ATP and undergo target-induced assembly on the chip surface and ZIF-8@MXene composites for their ability to provide a high surface density of aptamer-binding sites and facilitate the electron transfer at the biointerface. The SAE-nChip was capable of detecting ATP with a detection limit of 10 pM. Furthermore, this assay allowed the detection of ATP in cultured microorganisms and collected real bioaerosols. Overall, this strategy of interfacing DNA aptamers with MXene-based composite materials represents a versatile approach for the ubiquitous detection of biochemical targets in bioaerosols.
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