炎症体
链球菌溶血素
先天免疫系统
化脓性链球菌
促炎细胞因子
生物
微生物学
目标2
免疫系统
细菌
免疫学
炎症
细菌蛋白
金黄色葡萄球菌
遗传学
作者
Christine Valfridsson,Elsa Westerlund,Dóra Hancz,Jenny Persson
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 261-282
标识
DOI:10.1007/978-1-0716-3243-7_18
摘要
Inflammasomes are large multiprotein complexes that assemble mainly in innate immune cells after detection of microbial or sterile insults. Activation of inflammasomes is a key proinflammatory event during infection, and many pathogens have evolved specific evasion mechanisms to evade or inhibit inflammasome activation. One such pathogen is the common bacterium group A Streptococcus (GAS), which causes a wide range of diseases of varying severity. GAS secretes a multitude of virulence factors whereof the pore-forming protein streptolysin O (SLO) is the main inflammasome activation determinant. Here we provide a protocol for reliable evaluation of inflammasome activation in murine bone marrow-derived macrophages (BMDM) infected with GAS, including instructions for generating BMDMs and growing the bacterium. This protocol can easily be modified to other bacterial pathogens, or human macrophages.
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