黄芩
黄芩苷
类黄酮
转录因子
拟南芥
细胞生物学
类黄酮生物合成
拟南芥
西力克
GCLC公司
代谢物
白藜芦醇
发起人
抄写(语言学)
生物
转基因
下调和上调
Cis监管模块
转录调控
激活剂(遗传学)
查尔酮合酶
代谢途径
耐旱性
亚细胞定位
侧根
生物合成
转化(遗传学)
遗传学
基因表达调控
生物化学
转录组
作者
Chong Chen,Xiaofan Zhou,Bo Cao,Shan Feng,Tiantian Bin,Yali Zhang,Pufan Gao,Yumeng Lu,Xian Li,Lianjin Liu,Suying Hu,Bowen Zheng,Guishuang Li,Chengke Bai
摘要
Drought stress dynamically reprograms specialised metabolism in medicinal plants. However, the transcriptional regulatory modules governing stress-adaptive metabolite synthesis remain poorly characterised. Here, we identified SbMYB8 as a drought-responsive transcription factor showing nuclear localisation and dose-dependent induction under drought in Scutellaria baicalensis. SbMYB8 activation triggered coordinated upregulation of six baicalin biosynthetic genes, elevating total baicalin and aglycones. Heterologous overexpression in Arabidopsis thaliana revealed SbMYB8's conserved regulatory function, driving anthocyanin accumulation (2.3-fold), flavonoid hyperproduction (5.8-fold), and developmental plasticity through enhanced lateral root proliferation. Specifically, we established the first stable S. baicalensis genetic transformation system, enabling tissue-specific dissection of SbMYB8 function. Transgenic OE-SbMYB8 lines exhibited root architectural remodelling (thickened primary roots, increased lateral root density) and root-specific flavonoid amplification (baicalin 1.8-fold; total flavonoids 3.5-fold), coupled with hierarchical induction of 12 pathway genes. Low-dose PEG (2.5%) synergised with SbMYB8 to transiently boost aglycone synthesis, whereas high-dose stress (5%) disrupted this coordination, suppressing biosynthetic machinery and metabolite yields. Mechanistically, yeast one-hybrid and dual-luciferase assays revealed SbMYB8 directly binds cis-elements in target promoters to orchestrate pathway activation. Based on the above results, we propose a SbMYB8-mediated "drought perception - transcriptional activation - metabolic response" network and provide transformative tools for precision breeding of stress-resilient medicinal plants.
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