Glycyrrhiza uralensis crude water extract and licochalcone A and B to enhance chicken muscle satellite cell differentiation for cultured meat production

Muscle satellite cells (MSCs) are the most commonly used cells in cultured meat research and development. Enhancing MSC proliferation and differentiation while reducing cell culture costs is requisite to commercializing cultured meat. This study explored the effects of Glycyrrhiza uralensis crude water extract (GU-CWE) and licochalcone A and B (Lic A or B) on the proliferation and differentiation of chicken, bovine, and porcine MSCs. While GU-CWE and Lic A and B had negligible effects on bovine and porcine MSCs, GU-CWE significantly enhanced chicken MSC differentiation, and Lic A and B promoted both the proliferation and differentiation of chicken MSCs. Furthermore, GU-CWE was found to mitigate reactive oxygen species activity during chicken MSC differentiation and promote cell proliferation and adhesion in spheroid culture, thereby maintaining a spherical shape. Collectively, this study suggests that GU-CWE and Lic A and B can significantly reduce costs and safely increase the productivity of chicken MSCs in cultured meat production processes.