FOXP3型
流式细胞术
细胞凋亡
活力测定
转染
细胞周期
细胞
癌症研究
生物
下调和上调
细胞生物学
细胞培养
分子生物学
免疫学
化学
免疫系统
基因
生物化学
遗传学
作者
Jiangzhou Peng,Zigang Yu,Lei Xue,Jiabin Wang,Jun Li,Degang Liu,Qiang Yang,Yuan Lin
出处
期刊:Molecular Medicine Reports
[Spandidos Publications]
日期:2018-02-13
被引量:13
标识
DOI:10.3892/mmr.2018.8606
摘要
The aim of the present study was to investigate the novel mechanisms of forkhead box protein P3 (foxp3) in T regulatory (Treg) cells in lung cancer behavior. Treg cells were isolated from the peripheral blood of healthy volunteers and then co‑cultured with 95D cells. A plasmid overexpressing foxp3 was constructed and transfected into Treg cells and an MTS assay was performed to assess cell viability. Flow cytometry was performed to evaluate cell apoptosis and reverse transcription‑quantitative polymerase chain reaction was used to measure mRNA expression. A Transwell assay was used to assess cell invasion. Treg cells were successfully isolated from peripheral blood with purity of 94.26%. Foxp3 expression in Treg cells was significantly increased following co‑culture with 95D cells, while matrix metalloproteinase‑9 expression was upregulated in 95D cells co‑cultured with Treg cells. The apoptosis, invasion and migration abilities of 95D cells were suppressed by co‑culture with Treg cells, whereas the adhesive ability was enhanced. Foxp3 overexpression in Treg cells enhanced the viability and invasiveness of 95D cells, whereas cell adhesion and migration were decreased. The results of the present study demonstrate that the viability and invasiveness of 95D cells are enhanced by foxp3 overexpression in Treg cells, indicating that increased levels of foxp3 in the tumor microenvironment may promote tumor cell growth.
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