Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis elegans
基因
生物膜
囊性纤维化
细胞生物学
蜡螟
作者
Natalia V. Kirienko,Brent O. Cezairliyan,Frederick M. Ausubel,Jennifer R. Powell
出处
期刊:Methods of Molecular Biology日期:2014-01-01卷期号:1149: 653-669被引量:60
标识
DOI:10.1007/978-1-4939-0473-0_50
摘要
The nematode Caenorhabditis elegans is a simple model host for studying the interaction between bacterial pathogens such as Pseudomonas aeruginosa and the metazoan innate immune system. Powerful genetic and molecular tools in both C. elegans and P. aeruginosa facilitate the identification and analysis of bacterial virulence factors as well as host defense factors. Here we describe three different assays that use the C. elegans-P. aeruginosa strain PA14 host-pathogen system. Fast Killing is a toxin-mediated death that depends on a diffusible toxin produced by PA14 but not on live bacteria. Slow Killing is due to an active infection in which bacteria colonize the C. elegans intestinal lumen. Liquid Killing is designed for high-throughput screening of chemical libraries for anti-infective compounds. Each assay has unique features and, interestingly, the PA14 virulence factors involved in killing are different in each assay.