生物医学工程
组织工程
生物相容性
材料科学
体内
体外
骨组织
生物材料
化学工程
作者
Ramanathan Yegappan,Vignesh Selvaprithiviraj,Sivashanmugam Amirthalingam,Annapoorna Mohandas,Nathaniel S. Hwang,Rangasamy Jayakumar
标识
DOI:10.1016/j.ijbiomac.2018.10.182
摘要
Functional biomaterials that couple angiogenesis and osteogenesis processes are vital for bone tissue engineering and bone remodeling. Herein we developed an injectable carrageenan nanocomposite hydrogel incorporated with whitlockite nanoparticles and an angiogenic drug, dimethyloxallylglycine. Synthesized whitlockite nano particles and nanocomposite hydrogels were characterized using SEM, TEM, EDS and MR Developed hydrogels were injectable, mechanically stable, cytocompatible and has better protein adsorption. Incorporation of dimethyloxallylglycine resulted in initial burst release followed by sustained release for 7 days. Human umbilical vein endothelial cells exposed to dimethyloxallylglycine incorporated nanocomposite hydrogel showed enhanced cell migration and capillary tube-like structure formation. Osteogenic differentiation in rat adipose derived mesenchymal stem cells after 7 and 14 days revealed increased levels of alkaline phosphatase activity in vitro. Furthermore, cells exposed to nanocomposite hydrogel revealed enhanced protein expressions of RUNX2, COL and OPN. Overall, these results suggest that incorporation of whitlockite and dimethyloxallylglycine in carrageenan hydrogel promoted osteogenesis and angiogenesis in vitro. (C) 2018 Elsevier B.V. All rights reserved.
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