The effects of enzymatic treatment usi ng xylanase and laccase on the refining of natural bamboo fibers were studied.The chemical components and the linear density of bamboo fibers were evaluated.The experimental results show that the content of hemicelluloses or lignin decreases to a fixed value and then increases slowly with the increasing of enzyme concentration,and the content of hemicelluloses or lignin decreases with the extension of time,and the basic law of linear density of the natural bamboo fibers change reverses.The optimum conditions for xylanase:concentration of 0.5%,treatment time of 4 hours and temperature of 60 ℃,and the linear density of the fibers obtained reaches 5.82 tex,decreased by 42.9%;and those for laccase:concentration of 2.0%,treatment time of 4 hours and temperature of 60 ℃,and the linear density of the fibers obtained reaches 5.31 tex,decreased by 47.2%.