Determination of L-ascorbic Acid and D-isoascorbic Acid in Meat Products by High Performance Liquid Chromatography

The method was developed with the condition as follows:the sample was extracted with Metaphosphoric acid solution,HPLC analysis was performed on a Venusil MP C18 column with DAD detector,0.01moL/L KH2PO4 solution was used as mobile phase(pH 2.5~ 2.8) to establish a quick,accurate method of HPLC to determine L-ascorbic acid and D-isoascorbic acid in meat products.The results showed that the linear ranges were from 2 mg/L to 100 mg/L with correlation coefficients of 0.999.The mean recoveries were from 79.3% to 105.1% at concentration from 10 mg/kg to 100 mg/kg,and the RSDs were from 4.5% to 13.6%(n=6).It is concluded that this method is quick,accurate for determination of L-ascorbic acid and D-isoascorbic acid in meat products.