Amygdalin Used as the LC-ESI MS Internal Standard for Serum Multiple Constituents Quantitation After Drug Administration

Objective To investigate the feasibility of amygdalin being used as an LC-ESI MS internal standard for serum multiple constituents quantitation.Methods Serum samples spiked with amygdalin were mixed with trichloroacetic acid(TCA) for protein precipitation,separated by the gradient mobile phase of acetonitrile-water containing formic acid on Waters Acquity HSS T3 column,and detected in both the positive and negative mode after electrospray ionization(ESI).Results In both the positive and negative mode,good linear correlation was shown between the peak area and concentrationin serum amygdalin.When serum paeoniflorin and ferulic acid were determined in both the positive and negative mode with amygdalin as the internal standard(IS),good linear correlation was shown between the peak area ratio(test compound to IS) and the concentration of the test compound.Conclusion Amygdalin can be used as the LC-ESI MS internal standard for serum multiple constituents quantitation after drug administration.