Preliminary Optimization of Extraction Process of Rhus Chnensis Seed Protein

The content of protein fromRhus Chnensis seed was assayed by Kjeldahl determination and three protein extraction methods were employed.The results showed that the content of Rhus Chnensis seed protein was about 9.501%.Protein extracted by the improved acetone sedimentation method was higher than by the TCA-acetone extraction and basic extraction method.The optimum process conditions of improved acetone sedimentation method by orthogonal test were:the ratio of solid to liquid 1∶12g·mL-1,extraction time 3h,the ratio of protein extraction solution to acetone 1∶2,sedimentation time 1.5h.Practicing this optimal condition,the extraction rate of Rhus Chinensis protein reached 58.44%.The experiment data was referred for further study of the Rhus Chinensis seed protein.