Changes and clinical significance of CD4~+CD25~+Foxp3~+ regulatory T cell and IL-10 , TGF-β_1 in steroidresistant asthma patients of peripheral blood

Objective To investigate the changes of CD4+ CD25+ Foxp3+ regulatory T cell and IL-10,TGF-β1 in steroid-resistant asthma patients of peripheral blood,to study their significance.Methods The CD4+ CD25+ Foxp3+ regulatory T cell in 40 cases steroid-resistant asthma patients were detected by flow cytometry.The levels of serum IL-10 and TGF-β1 were detected by ELISA.The control groups were 30 individuals having general physical examination and 46 patients with steroid-sensitive patients.Results The proportion and number of CD4+ CD25+ Foxp3+ regulatory T cell and the levels of serum IL-10,TGF-β1 in steroid-resistant asthma group of Peripheral Blood were decreased significantly than steroid-sensitive group and healthy control group (P ＜0.01,P ＜0.05).The proportion and number of CD4+CD25+ Foxp3+ regulatory T cell and the levelsof serum TGF--β1 in steroid-sensitive group were decreased significantly than healthy control group,but the levels of serum IL-10 were no statistical difference between them.The proportion and number of CD4+ CD25+ Foxp3+ regulatory T cell were positively correlated with the serum IL-10,TGF-β1 (P ＜0.01).Conclusions Lower proportion and number of CD4+ CD25+ Foxp3+ regulatory T cell and lower IL-10,TGF-β1 were observed in steroid-resistant asthma patients,which might play an important role in occurrence and development of steroid-resistant asthma. Key words: Steroid-resistant asthma; Regulatory T cell; Transforming growth factor-β1; Interleukin-10