The phenotype of independent homozygous transgenic strains of glutamyl tRNA synthetase in Arabidopsis (Arabidopsis glutamyl tRNA synthetase, or AtGluRS) has been analyzed, including three over-expressed (GS-3, GS4, GS-6) and three inhibit-expressed (GAS2, GAS3, GAS7). It was showed that comparing to inhibit-expressed strains, plants with over-expressed AtGluRS were flowering later, growing faster and having more rosette leaves. In addition, the germination rate and growth analysis of AtGluRS transgenic plants under salt stress with sodium chloride (NaCl) reflected that over-expressing AtGluRS could enhance the resistance of plants to salt stress. Therefore, glutamyl tRNA synthetase in Arabidopsis not only affected plants phenotype, but also was involved in the process of abiotic stress tolerance.