The preparation of ~(99m)Tc-lactose-chitasan nanoparticles and analysis of its biodistribution for hepatic receptor imaging in mice

OBJECTIVETo prepare the chitosan-lactose acid complexes(99mTc-LA-CS)labeled by 99mTc, and to investigate the biodistribution of 99mTc-LA-CS in normal nude mice and the targeting effect of hepatocyte mediated by asialoglycoprotein receptor(ASGP-R).METHODS The 99mTc-LA-CS complexes were constructed by the stannous chloride reduction method,and were injectied to the nude mice tail vein, and then the mice were sacrificed at 10min,60min and 2h.It was collected that the blood,liver,spleen, stomach,intestine,kidney,lung and muscle tissues to be detected the 99mTc radiation capability,and the radioactive uptake rate of per gram of tissue from different organs(%ID/g)were calculated and the biodistribution in vivo was analyzed.RESULTS 99mTc labeling ratio was more than 93%,and the biodistribution results showed that high uptake was in the liver in nude mice,and the liver uptake rate was more than 70%ID/g at 60min.CONCLUSIONRadionuclide labeled 99mTc LA-CS has specific uptake in liver cells,and it is indicated that the 99mTc-LA-CS can be used for liver cell imaging as liver imaging agent mediated by ASGP-R.