The Application of SAM and SAH in Mice Tissues in Evaluation of Atherosclerosis

Objective Using high performance liquid chromatography(HPLC) to detect the changes of S-adenosyl-homocysteine(SAH) and S-adenosyl methionine(SAM),and to investigate its application in the evaluation of atherosclerosis(AS) in apolipoprotein E-knockout(ApoE-/-) mice.Methods SAM and SAH content changes in the hearts,livers and kidneys tissues of ApoE-/- mice groups(model control group,high-methionine diet group,folic acid and vitamin B12 treatment group) were detected and compared.Pathological changes of aorta in each group were observed by HE staining with light microscope.Results The results of HPLC showed that SAM,SAH levels in the normal control group,model control group and high-methionine diet group mice's hearts,livers and kidneys tissues were increased gradually.The SAM and SAH levels in the treatment group of ApoE-/- mice's hearts,livers and kidneys tissues were decreased.There were significant differences compared with the normal control group(P0.05).Compared with the model group,the differences of SAM and SAH contents in kidneys of high methionine diet group were significant(P0.05),the contents of SAM and SAH in livers and kidneys of treatment group were significantly different(P0.01).Compared with the high methionine diet group,there were significant difference of the SAM and SAH levels in livers,kidneys of treatment group(P0.01).Meanwhile,HE staining showed that AS pathological changes of aorta were significant in high-methionine diet group.Conclusion The increased expression of SAM,SAH may be one of the mechanisms that promoting the occurrence of atherosclerosis in the high-methionine diet ApoE-/- mice.