A Droplet-Free and Oil-Free Microfluidics Based on Self-Pumping Hydrogel for Point-of-Care Multiplex Digital Nucleic Acid Detection

in a single assay within 35 min on-site. This self-pumping hydrogel chip offers a simple, low-cost, and scalable platform that advances digital nucleic acid analysis toward portable and high-throughput point-of-care testing.