Overview of Protein Expression in Saccharomyces cerevisiae

Abstract This overview presents vectors and host strains that are available to direct gene expression in S. cerevisiae , including information on promoters, vector maintenance and copy number, transcription terminators, and selectable markers. Challenges to the expression of foreign proteins are also covered, including attainment of desired production yield, production of protein with appropriate post‐translational modifications, conformation and function, and secretion to the extracellular medium.