The X-ray crystal structure of formaldehyde dismutase at 2.3 Å resolution

Formaldehyde dismutase (FDM) exhibits the catalysis of the dismutation of aldehydes and alcohol: aldehyde oxdoreduction in the absence of an exogenous electron acceptor.The crystal structure of FDM was determined at 2.3 Å resolution by multi-wavelength anomalous dispersion method.FDM is composed of four identical subunits with a molecular weight of 44,000.Each subunit contains 2 zinc atoms.Preliminary intensity data were collected using CuKα radiation from a rotating-anode X-ray generator (Rigaku ultraX 18HF) operated at 40kV 100mA.Crystals of FDM belong to the space group I41 with unit-cell dimensions of a = b = 89.74Å, c = 226.77Å and there are two molecules per asymetric unit.Intensity data of native crystal and anomalous dispersion data were collected using Synchrotron Radiation at SPring-8 BL38B1.Experimental phases were obtained with MAD method using 3 wavelengths.