质粒
遗传增强
DNA
分子生物学
基因表达
生物
基因
裸DNA
化学
生物化学
作者
Guofeng Zhang,Vladimir G. Budker,Jon A. Wolff
出处
期刊:Human Gene Therapy
[Mary Ann Liebert]
日期:1999-07-01
卷期号:10 (10): 1735-1737
被引量:893
标识
DOI:10.1089/10430349950017734
摘要
We have previously shown that the intramuscular injection of naked plasmid DNA enables foreign gene expression in muscle. Further studies showed that the intravascular delivery of naked plasmid DNA enables high levels of expression not only in muscle but also in hepatocytes. For the liver, this technique required injection directly into the liver vessels (portal vein, hepatic vein, or bile duct) and occlusion of outflow. The present study now demonstrates that high levels of plasmid DNA expression in hepatocytes can be easily obtained by tail vein injections. The highest levels of expression are achieved by rapidly injecting the plasmid DNA in large volumes, approximately 2.5 ml. This technique has great potential for a wide variety of laboratory studies.
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