Super-resolution optical fluctuation imaging with high spatiotemporal resolution

Super-resolution microscopy opens up a new era for visualizing the dynamic subcellular structure and interaction in a whole new scale[1]. The methods to achieve super-resolution can be largely divided into two categories: (1) targeted modulation; and (2) stochastic modulation/fluctuation analysis. In stochastic single-molecule localization microscopy, the spatial resolution is gained through the scarification of temporal resolution, to ensure the sufficient sampling of the specimen. However, in super-resolution microscopy, the high spatial resolution is generally at a price of the imaging speed[2]. Super-resolution optical fluctuation imaging (SOFI) is a new technique which can allow multiple emitters within one diffraction limited region to be at on state simultaneously, and using the fluctuation dynamics to achieve super-resolution. Thus, it provides high spatial and temporal resolution simultaneously.