西玉米根虫
原位杂交
生物
原位
核糖核酸
信使核糖核酸
细胞生物学
植物
基因
遗传学
有害生物分析
化学
有机化学
出处
期刊:PubMed
日期:2022-01-01
卷期号:2360: 59-74
标识
DOI:10.1007/978-1-0716-1633-8_6
摘要
In situ hybridization (ISH) is a methodology by which nucleic acids are detected within fixed tissue samples. Recent advances in detection technology and target recovery have greatly enhanced the technique's ability to detect single mRNA molecules. Here we detail the fixation, paraffin embedding, sectioning, target recovery, and chromogenic detection of an mRNA (DvSSJ1), encoding for a membrane protein associated with the smooth septate junction (SSJ) in Western corn rootworm [Diabrotica virgifera (Dv)]. Further, we demonstrate, the expression of dsRNA of DvSSJ1 in maize root tissues using signal amplification and background suppression technology.
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