慢性阻塞性肺病
失调
肠道菌群
微生物群
粪便
免疫学
发病机制
肺移植
医学
肺
短链脂肪酸
生物
移植
生理学
内科学
微生物学
丁酸盐
生物信息学
发酵
食品科学
作者
Naijian Li,Zhouli Dai,Zhang Wang,Zhishan Deng,Jiahuan Zhang,Jinding Pu,Weitao Cao,Tianhui Pan,Yumin Zhou,Zhaowei Yang,Jing Li,Bing Li,Pixin Ran
标识
DOI:10.1186/s12931-021-01872-z
摘要
Dysbiosis of the gut microbiome is involved in the pathogenesis of various diseases, but the contribution of gut microbes to the progression of chronic obstructive pulmonary disease (COPD) is still poorly understood.We carried out 16S rRNA gene sequencing and short-chain fatty acid analyses in stool samples from a cohort of 73 healthy controls, 67 patients with COPD of GOLD stages I and II severity, and 32 patients with COPD of GOLD stages III and IV severity. Fecal microbiota from the three groups were then inoculated into recipient mice for a total of 14 times in 28 days to induce pulmonary changes. Furthermore, fecal microbiota from the three groups were inoculated into mice exposed to smoke from biomass fuel to induce COPD-like changes.We observed that the gut microbiome of COPD patients varied from that of healthy controls and was characterized by a distinct overall microbial diversity and composition, a Prevotella-dominated gut enterotype and lower levels of short-chain fatty acids. After 28 days of fecal transplantation from COPD patients, recipient mice exhibited elevated lung inflammation. Moreover, when mice were under both fecal transplantation and biomass fuel smoke exposure for a total of 20 weeks, accelerated declines in lung function, severe emphysematous changes, airway remodeling and mucus hypersecretion were observed.These data demonstrate that altered gut microbiota in COPD patients is associated with disease progression in mice model.
科研通智能强力驱动
Strongly Powered by AbleSci AI